Phage Fab Display Selection in Vitro and In Vivo: Novel Means to Identify New Breast Cancer Avid Compounds
Abstract
Breast cancer is the number one cause of death amongst cancer in women. New methods for early detection diagnosis and treatment of cancer are always sought. In this annual report we discuss the initial findings from in vivo affinity selection where we have sought to isolate Fabs that bind tumor material in tumor-bearing mice. Breast tumor cell line T47-D was used to produce xenografts in female SCID mice. Mice were injected with Fab-phage library and phage allowed to circulate for one hour. Tumor material was removed and binding phage isolated for amplification as the input for a subsequent round of in vivo biopanning. Five rounds were achieved. Fabs have been isolated from the final round and the DNA encoding them is being sequenced. Several Fabs will be assessed for binding to tumor material and extracted proteins and may be further mutated or engineering to incorporate radiometal atom binding sites for detection and imaging of primary tumors and metastases both in vitro and in vivo settings.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2002
- Accession Number
- ADA404909
Entities
People
- Mark A. Meighan
Organizations
- University of Missouri