Cell Type-Specific mRNA Amplification and Expression Profiling from Breast Tumor Sections
Abstract
The evolution of solid tumors involves acquisition of genetic abnormalities, which result in changes in both the set of genes expressed and the relative levels of gene expression. However, the increasing number of candidate genes whose expression needs to be evaluated for prognostic, diagnostic, therapeutic, or research purposes will require obtaining material from numerous tissue sections. Therefore this proposal is motivated by the need for more effective use of clinical specimens, and will address the problem of obtaining sufficient and cell type specific mRNA from clinical breast tumor specimens. This will entail adaptingideveloping procedures to amplify with fidelity the mRNA repertoire expressed in small numbers of normal, pre-cancerous and malignant breast epithelia. To this end, in this project period, we have concentrated effort on establishing and validating microarray-based assays for measuring gene expression levels and have demonstrated the capability to isolate and amplify mRNA from cultured cells. Realization of these objectives will allow, in the future, development of a resource, consisting of amplified mRNA populations from individual cells from normal and tumor material, that can be used for evaluation of the prognostic, diagnostic and/or therapeutic importance of genes expressed in breast cancer.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 2001
- Accession Number
- ADA405268
Entities
People
- Donna G. Albertson
Organizations
- University of California, San Francisco