Characterization of a p53 Regulatory Domain

Abstract

A putative domain may be present within p53 involving its regulation. Further evidence of a regulatory domain is derived from a comparison of p53 with two oncogenes, c-jun and c-fos. Amino acids 93-160 from p53 show a significant homology to c-jun and c-fos inhibitory domains, especially to c-jun's 8 region. The c-jun 8 region has been found to play an inhibitory role by interacting with a member of the. MAPK family, JNK. Once bound to c-jun, JNK is capable of phosphorylating serine residues 63 and %3. Two conserved serine residues within p53,s potential regulatory domain may also be involved in phosphorylation and regulation. Therefore, we hypothesized that p53 serine residues 116 and 127, homologous to c-jun serine residues 63 and 73, may be phosphorylated by JNK or another MAPK family member.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2001
Accession Number
ADA405271

Entities

People

  • Brian C. Abela
  • Kuan Liu

Organizations

  • University of California, Riverside

Tags

DTIC Thesaurus Topics

  • Amino Acids
  • Biomedical Research
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Chemistry
  • Degradation
  • Deoxyribonucleic Acids
  • Kinases
  • Laboratory Animals
  • Materials
  • Neoplasms
  • Phosphorylation
  • Proteins
  • Recombinant Dna
  • Regulations
  • Transcription Factors

Fields of Study

  • Biology

Readers

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  • Molecular and Cellular Biochemistry