Dendritic Cell-Targeted Phage Vectors for Breast Cancer Vaccine Development
Abstract
We hypothesize that one can use specific protein or peptide sequences to direct bacteriophage vectors to dendritic cells. We further propose that one can then use such re-targeted phage vectors to deliver potentially important antigens to dendritic cells, and that this may allow one to derive vectors capable of eliciting potent immune responses to breast cancer antigens such as her2. These hypotheses are being experimentally tested. During the period covered by this progress report, we have used phage display technology to identify peptide sequences which bind to cellular receptors expressed on dendritic cells, and we have conducted proof-of-concept studies to show that we can selectively target adenovirus vectors to dendritic cells using these peptide sequences. We have also shown that one can enhance intracellular internalization of T7 bacteriophage vectors using cell-binding peptides. We are now examining whether can use these same peptide sequences to deliver T7 and lambda phage vectors encoding a mammalian expression cassette, to primary dendritic cells. These experiments are expected to provide direct support for our hypothesis, that one can use phage vectors to express foreign genes in dendritic cells (including antigenic molecules, such as her2).
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2002
- Accession Number
- ADA405325
Entities
People
- Stephen Dewhurst
Organizations
- University of Rochester