Osteoblast-Prostate Cancer Cell Interaction in Prostate Cancer Bone Metastases
Abstract
Metastases from prostate cancer are characterized by their predilection for bone and their propensity to make more bone. This suggests that prostate cancer cells interact with cells from the osteoblastic lineage. To understand the molecular bases of prostatic bone metastases, we established two prostate cancer cell lines, MDA PCa 2a and MDA PCa 2b (1). These cell lines are derived from a bone metastasis of prostate cancer and have typical features of prostate cancer: they express prostate-specific antigen (PSA) and androgen receptor and their growth is regulated by androgen (1). Using these cell lines, we established and optimized an in vitro model of bone metastases from prostate cancer. In this system we co-culture prostate cancer cells with primary mouse osteoblasts (PMOs) in a Boyden chamber-type system so that the cells share medium but are not in physical contact (2). PMOs were isolated from the calvaria of 2- to 4-day- old CDI mice according to the procedure described in Yang et al (2). The PMOs were seeded in multiwell plate, and MDA PCa 2a and MDA PCa 2b cells were seeded in chamber inserts with an 0.4 pm pores (Falcon/Becton Dickinson Labware, Franklin Lakes, NJ). After 24 h of cell culture, the inserts containing MDA PCa 2a and MDA PCa 2b cells were placed into tissue-culture plates containing the PMOs so that the two different cell types shared the culture medium but were not in physical contact. As a growth medium we used alpha-minimal essential medium (alpha-MEM) plus 10% fetal bovine serum (FBS) plus 5% BRFF-HPCI medium (Biological Research Faculty and Facility, Inc., Jamsville, MD) (2)). In all cases PMOs and prostate cancer cells growing alone were used as controls. The medium was changed every 2 days. A similar method was used to co-culture LNCaP, PC3, HeLa and T24 cell lines with PMOs. LNCaP is derived from a lymph node metastasis of prostate cancer, not from a bone metastasis (3).
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2001
- Accession Number
- ADA405354
Entities
People
- Nora M. Navone
Organizations
- The University of Texas MD Anderson Cancer Center