Novel Technology for Cloning Prostate Cancer Cell Markers
Abstract
The purpose of the project is to employ probes isolated from the LNCaP series of human prostate cancer cells, to probe human cDNA microarrays, so as to investigate genes differentially expressed among these cell lines; prepare from the LnCaP series, particularly the C4-2B cells representing the most advance cancer stage, RNA corresponding to a purified "membrane fraction", expected to contain the mRNAs for cell surface markers or secretory proteins; and employ these probes to probe human cDNA microarrays, to identify genes whose products might form clinical assays for advanced prostate cancer. Work during the period covered included: (i) Incorporation of the powerful new DNA microarray technology into the project, and its use to investigate differential gene expression in the LNCaP series of human cell lines, representing different stages of prostate cancer. (ii) Studies finally yielding preparation of highly pure membrane fraction from these cells; but resulting in insufficient yield for preparation of DNA microarray probes.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 2002
- Accession Number
- ADA405355
Entities
People
- F. C. Bancroft
Organizations
- Mount Sinai Hospital