Identification of Novel Oncogenes in Carcinoma of the Prostate
Abstract
A cell line termed RK3E provides a novel approach to identification and functional analysis of carcinoma-derived transforming oncogenes. Derived by immortalization of primary rat kidney cells, RK3E exhibit multiple properties of epithelia and specifically detect the transforming activity a small subset of all oncogenes. The Aim of this proposal was to identify novel transforming activities in prostatic carcinoma by expression cloning in RK3E cells. This was not completed, in part due to routine technical problems encountered during cDNA library construction. Instead, we analyzed expression of GKLF, recently identified as a novel RK3E transforming oncogene, in several human tumor types. GKLF was found to be expressed at elevated levels in oral squamous cell carcinoma (100%) and in breast carcinoma (70%) in vivo. However, expression was reduced or unaltered in prostatic carcinoma. Thus, these results appear to exclude a role for GKLF in progression of prostatic carcinoma. The results suggest that carcinoma types for which oncogenes are poorly characterized, including prostatic carcinoma, can be rapidly assessed for novel transforming activities, and that genes identified by this in vitro assay are likely to be activated during tumor progression in vivo.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 2001
- Accession Number
- ADA405358
Entities
People
- John M. Ruppert
Organizations
- University of Alabama