USF-1 as an Inhibitor of Mammary Gland Carcinogenesis
Abstract
The hypothesis tested in this proposal is that overexpression of USF in the mammary glands of transgenic mice will inhibit myc-dependent tumorigenesis. To test this hypothesis, a transgene was constructed to target the overexpression of PLAG-tagged USF-2 to the mammary glands of transgenic mice under the control of the mouse mammary tumor virus (mmtv) long terminal repeat. A total of eight lines of transgenic mice were generated. Of these, one line demonstrated expression of flag-tagged USF-2 in the lactating mammary gland at levels 12-fold over that of endogenous USF-2. Analysis of mammary gland development and lactation in these mmtv-USF-2 transgenic mice supports the conclusion that overexpression of USF-2 has minimal impact. To determine the impact of USF-2 on myc-dependent mammary tumorigenesis, progeny from genetic crosses of the mmtv-USF-2 mice with transgenic mice that overexpress either c-myc or Ha-ras are currently being generated. Preliminary evaluation of tumorigenesis in these mice suggests that USF-2 by itself is not oncogenic. Analysis of tumorigenesis in larger populations of these transgenic mice will be completed in the coming year and will establish if mmtv-USF-2 can block myc-dependent tumorigenesis.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2001
- Accession Number
- ADA405501
Entities
People
- Darryl L. Hadsell
Organizations
- Baylor College of Medicine