Selective DNA Delivery to Breast Cancer Cells
Abstract
The hypothesis of this research is that specific cell-binding proteins or peptides can be used to enhance (therapeutic) DNA delivery to breast carcinomas; this hypothesis is being experimentally tested. During the period covered by this progress report, we have continued our analyses of the novel alpha V beta 3-binding proteins which we first described in our previous annual report. These were developed by the directed mutagenesis of a natural integrin-binding protein, the tenth fibronectin type III domain (FNfn10), and subsequent screening of a library of these molecules, using phage display technology. A novel derivative of FNfn10 was identified and shown to bind with high affinity and specificity to purified alpha V beta 3 integrin. It also interacted with cell surface-expressed alpha V beta 3, as determined by flow cytometry, but did not bind detectably to other cell surface integrins. Overall, these experiments have provided important tools and insights that will enhance gene transfer to breast carcinomas.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2002
- Accession Number
- ADA405504
Entities
People
- Stephen Dewhurst
Organizations
- University of Rochester