Functional Analysis of C-CAM Tumor Suppressor Gene by Targeted Gene Deletion
Abstract
C-CAM1 (also named Ceacam1) is a cell adhesion molecule of the immunoglobulin supergene family. We have shown that C-CAM1 plays critical roles in prostate cancer initiation and progression and that loss of C-CAM1 is an early event in the development of prostate cancer. Although tumorigenesis studies in mouse xenograft model have suggested the involvement of C-CAMl in epithelial cell growth and differentiation, the fictional roles of C-CAMl in normal prostate development, prostate homeostasis, and prostate tumorigenesis remain unclear. We propose to determine the roles of C-CAMl's growth suppressive activity in prostate growth and tumorigenesis by using gene targeting and embryonic stem cell technologies to generate C-CAMl knockout mice. We have designed a gene targeting strategy that is specific to Ceacam1 gene. In addition, the Ceacam 1 gene in the targeted vector was flanked by loxP sites to allow for generating both straight and conditional knockout of Ceacaml gene. The targeting vector has been constructed and 24 embryonic stem cell clones containing the recombinant gene allele have been established. Three of the embryonic cell clones have been injected into blastocysts for germ line transmission of the targeting construct.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2002
- Accession Number
- ADA406123
Entities
People
- Sue-hwa Lin
Organizations
- University of Houston