Prostatic Fluid Cells
Abstract
Most research that requires the long-term propagation of prostate cancer (PCA) cells in culture is carried out with three cell lines: DU 145, PC-3, and LNCaP. Ml of these lines were derived from metastases; only one, LNCaP, makes prostate specific antigen (PSA) and/or androgen receptor. Neither DU 145 nor PC-3 exhibit any phenotypic markers specific for prostatic epithelial cells. Better models are needed. There is evidence that some prostatic fluids contain PCA cells. For this proposal, our goals are (1) to test the tumorigenicity of and to develop transplantable xenografts from PCA cells in prostatic fluid, (2) to develop methods for enhancing the tumorigenicity of small numbers of these PCA cells without deliberately altering their genes, (3) to test these methods for enhancement of tumorigenicity with prostatic fluid cells, and (4) to initiate clinical follow-up. To date, we have detected a marginal elevation of PSA in the blood of one mouse that received prostatic fluid cells 28 days after injection, unequivocal elevation of PSA in the blood of two mice 2-4 months after transplantation, and no elevation of PSA 6 months after transplantation. We have studied the coinjection of lethally irradiated, growth-factor-producing cells from several tumors with encouraging results in the case of some tumors.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2002
- Accession Number
- ADA406134
Entities
People
- Thomas G. Pretlow
Organizations
- Case Western Reserve University