Silver Colloids in Bacteria: A Study by Transmission Electron Microscopy, Absorption Spectroscopy and Elemental Analysis

Abstract

Reduction of silver ions in and on bacterial cells was shown to produce silver colloids giving rise to intense surface enhanced Raman spectra (SERS) of chemical components of the cell. Transmission electron microscopy, absorption spectroscopy and elemental analysis were used to explore the distribution of silver in and on the cells produced by two different protocols. The first protocol gives rise to an internal colloid of nanometer sized silver particles uniformly distributed within the cells. The second protocol produces a rough (aggregated) silver layer on the cells surface. Both protocols largely preserve the shape of the cells although there is a size reduction, which is to be detailed in a subsequent report. Treatment with Potassium cyanide removes the silver and reverts the cells to a size and shape similar to the original.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2002
Accession Number
ADA406581

Entities

People

  • Burt V. Bronk
  • J. Czege
  • N. Faey
  • R. Herd
  • S. Efrima
  • Z. Z. Li

Organizations

  • Air Force Research Laboratory

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Absorption
  • Air Force
  • Bacteria
  • Cellular Structures
  • Chemistry
  • Composite Materials
  • Electron Microscopy
  • Electrons
  • Materials Science
  • Microscopy
  • Particles
  • Raman Spectra
  • Raman Spectroscopy
  • Scattering
  • Spectra
  • Spectroscopy
  • Transmission Electron Microscopy

Readers

  • Immunology and Pathology
  • Nanoscale Plasmonic Nanotechnology

Technology Areas

  • Microelectronics