Role of c-myb in Breast Development and Cancer
Abstract
C-MYB, ALONG WITH A-myb and B-myb, belongs to the myb gene family which codes for nuclear proteins that bind DNA in a sequence-specific manner and function as regulators of transcription. There is a large body of evidence to suggest a role for c-myb in breast development and cancer c-myb is highly expressed in all estrogen receptor positive breast tumors and cell lines. In addition, our in situ hybridization studies show that c-myb is expressed at high levels in ductal cells from breast tissues of virgin and pregnant mice. To address the role of c-myb in mammary development and cancer, we propose to create c-myb conditional knockout mice where the expression of this gene is interrupted specifically in the mammary gland using the Cre-lox system. We are in the process of generating chimeric mice by injecting our conditional c-myb deletion ES cells into blastocysts of C57 X CBA pseudopregnant mice. Founder mice will be identified and bred to homozygosity for carrying conditional c-myb deletion alleles and, additionally, to bear either a MMTV-cre or a WAP-cre transgene. The generation of breast-specific c-myb conditional knockout mice will afford us the opportunity to dissect the role of c-myb in normal breast development and cancer.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2002
- Accession Number
- ADA406816
Entities
People
- Yen K. Lieu
Organizations
- Temple University