Identification of Apoptosis Genes Induced by the Human Prolactin Antagonist, hPRL-G129R, in Several Breast Cancer Cell Lines
Abstract
We compared the differential gene expression profile of 4 human breast cancer cell lines following treatment with hPRL and its antagonist. Among the genes identified of an apoptosis array, the bcl-2 gene was of particular interest, and we found that this gene was upregulated in 3 of the 4 cell lines that were treated with PRL. To further confirm these results, real-time PCR analysis was used to detect bcl-2 mRNA, in 11 human breast cancer cell lines after treatment with hPRL or its antagonist. Our data suggest that Bc 1-2 is upregulated in response to hPRL stimulation & is competively inhibited by the antagonist in the majority of the cell lines tested. We propose that the anti-apoptotic role of hPRL in breast cancer is mediated, at least in part, through regulation of bcl-2.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 2002
- Accession Number
- ADA406839
Entities
People
- Susan K. Peirce
- Wen Y. Chen
Organizations
- Clemson University