Function of Etk in Growth Factor Receptor Signaling to Integrins in Breast Cancer
Abstract
The central hypothesis in this IDEA Award is that increased integrin-mediated adhesiveness and migration of breast cancer cells in response to stimulation by the growth factors heregulin-beta(HRG-beta) or epidermal growth factor is mediated by phosphoinositide 3-OH kinase (PI 3-K)-dependent activation and membrane recruitment of the novel Tec family tyrosine kinase Etk. During the past year, we have made progress in several aspects of the initial Statement of Work, including: 1) establishment of a correlation between Etk expression and several breast tumor cell lines with high migratory capacity; 2) demonstration of HRG-beta-dependent increases in tyrosine phosphorylation of Etk in breast cancer cells; 3) demonstration of binding of the PH domain of Etk to the PI 3-K lipid product PI(3,4,5)-P3; 4) demonstration that HRG-beta stimulation leads to enhanced membrane localization of Etk that requires the PH domain of Etk and PI 3-K activity; and 5) production and establishment of molecular reagents and methodologies critical to completion of the Statement of Work.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 2002
- Accession Number
- ADA406853
Entities
People
- Yoji Shimizu
Organizations
- University of Minnesota