P16 Axis in Androgen-Dependent Proliferation of Prostate Cancer Cells

Abstract

The purpose of this study is to understand the role of the p16 growth control axis in androgen dependent proliferation of prostate cancer cells. The p16 axis contains two tumor suppressors (plGInk4a and pRB), cyclin D-dependent kinases, and transcription factor E2F. We hypothesized that functions of the p16 axis can influence androgen-dependence of prostate cancer cells. To test this hypothesis, we proposed to use controlled expression techniques to determine whether disruption of p16 axis function can lead to androgen- independence in human androgen-dependent prostate cancer cells (LNCaP) and, on the other hand, whether restoration of p16 axis function can restore androgen-dependence in androgen-independent prostate cancer cells (DU-145). During the second year of this study, we have established LNCaP derivative cell lines that can provide inducible expression of exogenously introduced proteins. In DU-145 cells, we have discovered that restoration of protein expression of pRB and the androgen receptor cause cell death. This finding reveals a novel functional relationship between pRB and the AR in prostate cancer biology.

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Document Details

Document Type
Technical Report
Publication Date
Apr 01, 2002
Accession Number
ADA406854

Entities

People

  • Liang Zhu

Organizations

  • Albert Einstein College of Medicine

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Androgen Receptors
  • Androgens
  • Anti-Bacterial Agents
  • Apoptosis
  • Biomedical Research
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Epithelial Cells
  • Neoplasms
  • New York
  • Phase Transformations
  • Prostate
  • Prostate Cancer
  • Proteins
  • Regulations

Readers

  • Molecular Biology and Genetics
  • Prostate Cancer Biology.