Pain Transmission in Humans: The Role of Novel Sensory Ion Channels
Abstract
The primary accomplishments of the previous funding period were: 1) RACE analysis of the 5' end of Navl.8/Scn10a murine DRG aDNA confirmed the presence of one intron splice site in the 200 bp 5'UTR of the Scn10a transcript; 2) Three BAC clones were isolated from a genomic library. All three contain at least the entire cds containing portion of the Scn10a gene plus the 5'UTR and an additional >4.0kb of upstream sequence; 3) Homologous recombination (conversion) of all three BAC clones to EGFP containing reporter constructs has been completed; 4) LM-PCR upstream from the putative transcription start site (5'RACE end) produced 4.0kb of sequence that may contain some/all of the elements required for the exquisitely controlled expression of Navl.S, the Scn10a gene product; 5) The putative promoter appears to contain several known consensus binding sequences for transcription factors; 6) Putative neuronal-specific silencer elements may be present. Deletion analysis of the 4 kb promoter fragment indicates activating elements between -3200 and -2500 bp. These latest findings will allow us to extend our analysis of the regulation of the Scn10a gene and efficiently focus our efforts during the final year of this project.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 2002
- Accession Number
- ADA406960
Entities
People
- Henry L. Puhl Iii
- John Noti
- Robert S. Aronstam
- Stephen R. Ikeda