Recombinant Antibodies Specific to Bacillus Anthracis Spores
Abstract
Recombinant antibody and DNA technologies have been used to improve the sensitivity and specificity of rapid assays for the detection of B. anthracis spores. We have identified a spore coat gene, cotK, which demonstrates substantial sequence variation among B. anthracis clade members including B. cereus, B. thuringiensis and B. mycoides strains. A central 25 amino acid region of the CotK protein was identified that varies in sequence among the B. anthracis clade strains analyzed. Peptides were synthesized containing the 2% residue CotK variable region from B. cereus, B. thuringiensis and B. anthracis strains. These peptides, when coupled with a carrier conjugate, were shown to produce antibody responses in animal models.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 30, 2002
- Accession Number
- ADA406971
Entities
People
- Terrance Leighton
Organizations
- University of California, Berkeley