Transcriptional Regulation of VEGF Expression in Breast Cancer
Abstract
Increased levels of VEGF protein in breast cancer correlate with poor prognosis in the disease. Little is currently known about the transcriptional activation of the gene in breast cancer, and the regulation could be different from other cancer types. To identify promoter segments that contribute to enhanced expression of VEGF in breast cancer, 10 reporter constructs encompassing nested deletions of the wild type-promoter from positions -1200 to -50 were synthesized and screened for activity in human MCF-7 and MDA-MB231 breast cancer cells. Two segments between positions -1200 and -1000 and -270 and -140 were identified as contributors to transcriptional activation of the promoter in these cell lines. Gel shifts using nuclear extracts from MCF-7 and MDA-MB231 cells further indicated differences in transcription factor binding to the proximal promoter element that drives basal expression between positions -B8 and -65.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2002
- Accession Number
- ADA407270
Entities
People
- Sebastian Bredow
Organizations
- Lovelace Foundation