Targeting of Prostate Cancer With Hyaluronon-Binding Proteins
Abstract
To test our hypothesis that the hyaluronan (HA) binding proteins (HABP) from cartilage is a new category of anti-tumor agents, we have proposed to focus on three aims: 1) To examine the effect of HABP on the tumor growth of prostate cancer cell lines; 2) To examine the effect of link protein and aggrecan on the tumor growth of prostate cancer; 3) To examine the possible anti-angiogenesis effect of HABPs. In past year, we have successfully finished the following works: 1) cloned 993 bp of cDNA cording for N-terminus of human aggrecan, which consists of at least six HA binding motifs; 2) inserted the cDNA into yeast expression vector; 3) purified the recombinant human aggrecan for the media of transformed yeast; 4) characterized that this recombinant aggrecan was highly glycosalated; 5) demonstrated that the recombinant aggrecan could inhibit the proliferation of endothelial cells; and 6) demonstrated that the recombinant aggrecan could inhibit the tumor growth in vivo. We believe that this part of study is very meaningful: 1) it determines that the aggrecan is an anti-tumor element in cartilage; 2) the aggrecan in human form may be used directly in patients, if the effect is potent enough; 3) the cDNA cloned in this study may be used for gene therapy.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2002
- Accession Number
- ADA407277
Entities
People
- Lurong Zhang
Organizations
- Georgetown University