Synthetic Lethality in Breast Cancer Cells: Genes Required for Tumor Survival

Abstract

The conversion of a normal cell into a cancer cell proceeds through a series of genetic and epigenetic alterations. We have proposed to use well-established genetic methodologies to identify novel anti-cancer targets via their specific, genetic interactions with common cancer mutations. In short, we will identify genetic alterations that are neutral in normal cells, but that are lethal when combined with cancer mutations. This "synthetic lethality" approach may identify potential therapeutic targets that are highly specific to the cancer cell. In the past year, we have made substantial progress toward the goal of developing technologies necessary to making this type of target search a reality. We have devised genetically defined human cancer models that can be used to conduct synthetic lethality screens and have developed new methodologies for manipulating gene expression in mammalian cells.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2002
Accession Number
ADA407287

Entities

People

  • Gregory Hannon

Organizations

  • Cold Spring Harbor Laboratory

Tags

DTIC Thesaurus Topics

  • Breast Cancer
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Chromosomes
  • Gene Therapy
  • Genetic Engineering
  • Genetics
  • Health Services
  • Lymphocytes
  • Neoplasms
  • Proteins
  • Rna Stability
  • Stem Cells

Fields of Study

  • Biology

Readers

  • Molecular and Cellular Biochemistry
  • Oncology
  • Systems Analysis and Design

Technology Areas

  • Biotechnology