UG311, an Oncofetal Marker Lost With Prostate Cancer Progression
Abstract
The mRNA expression of a paralogous human sequence to UG311, a murine urogenital sinus expressed sequence tag, was found to decrease with androgen independent progression in the LNCaP model of human prostate cancer progression as determined by RNA blotting. Analysis of the sequence of UG311 determined significant homology to a single-stranded nucleic acid binding protein, nmt55. To exclude nmt55 as the gene corresponding to UG311, antibodies and cDNAs were acquired. Scope: As members of the single-stranded nucleic acid binding protein family, nmt55 and UG311 may play a role in DNA repair or RNA splicing. Both functions are currently under evaluation for nmt55. Either of these functions is likely to have impact on the progression and potential therapeutic outcome for prostate cancer patients. Improper splicing would lead to entire classes of proteins being disrupted. Loss of DNA repair enzymes would lead to increased genomic instability and accelerated progression. Major Findingslprogress: We have attempted to focus on the cloning of the UG311 paralog with a minor emphasis on completing the exclusion of nmt55 as the UG311 paralog. The UG311 insert was used as a high stringency probe to screen the C4-2 lambda ZAP library. Ten strong plaques after two rounds of purification were analyzed for the size and sequence of the insert acquired. All inserts matched to the NONO sequence. This is a candidate as it shows significant ho insert sequence. Unfortunately, this sequence shows an increase expression in the human prostate cancer cell lines as compared to previous data from 11.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2002
- Accession Number
- ADA407342
Entities
People
- Barbara Szmoju
- Brian E. Nicholson
- Henry F. Frierson Jr
- Margaret C. Shea
- Robert A. Sikes
Organizations
- University of Virginia