TGF-Beta and Breast Cancer Induction
Abstract
We attempted to generate mice that produced active TGF-Beta in the breast by the use of a transgene containing the WAP promoter driving the expression of a truncated form of the latent TGF-Beta binding protein (LTBP) . TCF-a is normally produced in a latent form and is part of a complex consisting of TGF-Beta, the TGP-Beta propeptides and (LTBP) . We had shown that the expression of this type of transgene using the Kl4 promoter resulted in the production of active TGP-Beta in the skin and the suppression of growth. We hypothesized that mice expressing this transgene would produce active TGP-Beta, which would block the growth of epithelial cells and suppress tumor induction if expressed in mice with a high incidence of mammary tumors. Mice were produced that expressed the appropriate transgene in the breast. However, no consistent effect on the development of the glandular system in the breast was observed at 9, 24 or 36 weeks of age. The reasons for this may relate to the limited temporal expression of the WAP promoter. The effect of the transgene in mice susceptible to mammary tumors was not tested because of the failure to observe an effect in control mammary gland development.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2002
- Accession Number
- ADA407399
Entities
People
- Branka B. Dabovic
- Daniel B. Rifkin
Organizations
- New York University