Vitronectin and Integrin alphanubeta3 in Ovarian Carcinoma
Abstract
One specific characteristic of ovarian cancer cells is their ability to survive in the absence of adhesion. Our previous studies demonstrate that the expression of vitronectin and alphavbeta3 integrin and the interaction between them are essential for ovarian cancer cell survival in suspension. We thus hypothesize that ovarian cancer malignancies may be suppressed by blocking vitronectin and integrin expression. In the present study, we employed a newly developed technology called RNA interference for inhibiting vitronectin and beta3 integrin expression. Four sets of short interfering RNA (siRNA) were designed for vitronectin and beta3 integrin subunit mRNA, and these siRNA were expressed in ovarian cancer cells using the pSUPER system. The expression of at least one vitronectin siRNA and one beta3 integrin siRNA significantly inhibited cellular vitronectin and alphavbeta3 integrin expression. Our studies demonstrate that specific and potent siRNA can be developed to suppress vitronectin and alphavbeta3 integrin expression in ovarian cancer cells.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2002
- Accession Number
- ADA407410
Entities
People
- Shuang Huang
Organizations
- Scripps Research