Multiparametric Evaluation of Marker Expression
Abstract
We have developed flow cytometric methods for the analysis of androgen and vitamin D receptor expression in nuclei isolated from archival breast tumors of both male and female patients. Our data shows that in male breast tumors androgen receptor expression is less than that of the female tumors. In both male and female tumors, sub-populations with diploid DNA content had lower receptor expression than in nuclei with aneuploid DNA content. In contrast the expression of vitamin D receptors in diploid and aneuploid tumors was similar. The methods developed are rapid, quantitative and allow for multiparametric analysis of receptor expression in archival breast tumors.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2002
- Accession Number
- ADA407422
Entities
People
- Awtar Ganju-krishan
- Wahrdad Nadji
Organizations
- University of Miami