Assessing the Association of Epstein-Barr Virus With Breast Carcinoma
Abstract
Epstein-Barr Virus (EBV) contributes causally to specific lymphomas and carcinomas in people. A recent retrospective survey detected EBV DNA in 51 of 100 consecutive breast carcinoma biopsies by PCR, in 3 of 30 normal adjacent biopsies, and a viral protein, EBNA-l, in a fraction of tumor cells in 9 of 9 EBV-positive biopsies assayed immunohistochemically (Bonnet et al., J. Nati. Cancer Inst. 91:1376-1381, 1999). These data reveal a statistically meaningful association of EBV with breast carcinoma which we shall examine further to confirm or refute. The EBV in positive biopsies is either in the carcinoma cells or in surrounding normal cells such as B-lymphoid cells, a normal site for EBV's latent infection. To assess this association we shall use fixed blocks of tissue for which pairs of one normal adjacent tissue and one breast carcinoma are available. The percent of carcinoma cells present in fixed blocks will be estimated to be less than 1%, from 1% to 10%, or greater than 10%, and the amount of EBV present per cell in all samples determined by multiplex PCR and glass microarrays to measure EBV DNA from known levels of isolated sample DNAs.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2002
- Accession Number
- ADA407441
Entities
People
- Bill Sugden
Organizations
- University of Wisconsin–Madison