Rb Associated Protein 46: Roles in Progression of Proliferative Breast Cancer
Abstract
Our research program is focused on the study of Rb associated protein 46 (RbAp46), a novel tumor suppressor gene we recently identified and cloned. Long term goal of this study is to understand the role of RbAp46 in abnormal growth of mammary epithelial cells, in particular, the effects of constitutive and high levels of RbAp46 expression on the early development of breast cancer. In the past year (months 13-24), we have finished in vivo xenograft experiments proposed for months 1-12. Furthermore, we have established stable cell lines that express exogenous RbAp46 from human breast cancer cells, MCF7, MDA-MB-231 and MDA-MB-436, and found that high levels of RbAp46 expression strongly inhibit malignant features of these breast cancer cells. We have also found that expression levels of beta-catenin, an important signaling molecule, were downregulated in RbAp46-transfected cells compared with control cells. The lower levels of t3-catenin were restored by treatment of cells with proteasome inhibitors. We further discovered that the levels of GSK-3 beta expression were upregulated and beta-catenin/Tcf-mediated transcription pathway was strongly downregulated in RbAp46-transfected cells.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2002
- Accession Number
- ADA407498
Entities
People
- Guan C. Li
- Zhao Y. Wang
Organizations
- Beth Israel Deaconess Medical Center