Crystal and Solution Structure of the Photoprotein Obelin
Abstract
The three-dimensional structures of several types of recombinant obelin have been determined to atomic resolution. Obelin is a calcium-regulated photoprotein and the origin of the bioluminescence from the marine hydroid polyp Obelia. A W92F mutant obtained showed a violet bioluminescence emission but without change in dimensionality of the substrate binding site. The structures are typical of the super-family of calcium-binding E-F hand proteins. The high quality of the crystals also allowed a novel crystal lographic method of anomalous scattering from the protein sulfur, to be demonstrated. The substrate coelenterazine is bound within the protein substituted as a 2-hydroperoxide. The NMR study indicated that the solution secondary structure did not differ substantially from the crystal.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 17, 2002
- Accession Number
- ADA407919
Entities
People
- John K Lee
Organizations
- University of Georgia