Estrogens and Breast Cancer

Abstract

In the present work we demonstrate that estradiol and its metabolites mainly 4-OH estradiol are able to induce transformation phenotypes in the human breast epithelial cells (HBEC) MCF-10F. This cell line is a spontaneously immortalized HBEC that does not contain measurable level of ER alpha or ER beta. Short-term treatment of these cells with physiological doses of 17-beta estradiol induces anchorage independent growth, colony formation in agar methocel, and reduces ductulogenic capacity in collagen gel, all phenotypes whose expression is indicative of neoplastic transformation. Physiological doses of 4-OH-E2 (0.007nM) induces transformation phenotypes that are not abrogated by the pure antiestrogen ICI.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2002
Accession Number
ADA408065

Entities

People

  • Jose Russo
  • Yun-fu Hu

Organizations

  • Fox Chase Cancer Center

Tags

DTIC Thesaurus Topics

  • Breast Cancer
  • Cancer
  • Carcinoma
  • Cell Physiological Processes
  • Cells
  • Cellular Structures
  • Chemical Synthesis
  • Chemistry
  • Chromosomes
  • Diseases And Disorders
  • Epithelial Cells
  • Genetics
  • Neoplasms
  • Oncology
  • Skin Diseases

Fields of Study

  • Biology

Readers

  • Breast cancer cell signaling and growth regulation.