Functional Interactions of the TACC2 Breast Tumor Suppressor Gene and Its Relevance to Breast Tumor Progression
Abstract
Recently, in the HMT-3522 cell line based model for breast tumor progression, TACC2 mRNA was shown to be downregulated in the more malignant clones of the series. This indicates that TACC2 downregulation is an important step in breast tumor progression. We have now shown that increased expression of TACC2 alters the in vitro cellular dynamics of breast cancer cell lines in a cell type specific manner. While TACC2 expression does not appear to inhibit cellular division, TACC2 does affect anchorage independent growth and cell migration. We have demonstrated that TACC2 interacts with hGCNS, a key component of transcriptional regulatory complexes. This suggests that TACC2 could play a role in the regulation of transcription through interaction with this molecule. The observation of specific effects of TACC2 expression in estrogen receptor negative compared to estrogen receptor positive cell lines may also support this idea. Therefore, further characterization of the role of the interaction of TACC2 with hGCN5 in transcription will elucidate how alterations in this complex could promote the malignant phenotype.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2002
- Accession Number
- ADA408097
Entities
People
- Ivan H. Still
Organizations
- Health Research, Incorporated