BRCA2 and Genome Integrity
Abstract
The BRCA2 tumor suppressor gene has been suggested to play an important role in DNA repair and maintaining genome integrity. This suggestion, however, is mainly based on results obtained from studying mouse embryonic cells. The importance of BRCA2 in maintaining genome integrity of human somatic cells is not very clear. We have completed the Task 1, generation of Capan-1 derivatives that conditionally express wild type BRCA2. We have also carried out the first part of the Task 3, characterization of Capan-1 derivatives to genotoxic agents. Our results showed that there was no detectable difference in the sensitivity to radiation and DNA damaging chemicals between Capan-1 cells that express the wild-type BRCA2 and those do not. We have performed Task 2, generation of MCF7 and MCF-12A derivatives that do not express BRCA2. We have attempted the antisense cDNA approach but did not obtain any clone that expressed reduced level of BRCA2. In order to disrupt the BRCA2 gene, we have constructed a somatic gene-targeting plasmid.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2002
- Accession Number
- ADA408131
Entities
People
- Li-kuo Su
Organizations
- The University of Texas MD Anderson Cancer Center