Caspase Deficiency: Involvement in Breast Cancer Carcinogenesis and Resistance
Abstract
This project was proposed to study the correlation between caspase deficiency and breast cancer carcinogenesis and therapeutic resistance. During the third year, we continued to perform the functional screening of breast cancer cell lines treated with TNF-alpha and correlate their response with expression status of caspases in the cell lines detected in specific aim 1. Among the 7 breast cancer cell lines detected this year, BT-483, MDA-NB- 134VI and MDA-MB-415 cells, which expressed lower levels of caspase 8, were resistant to TNF-alpha. When 19 breast cancer cell lines were treated with granzyme B, correlation between granzyme B induced apoptosis and caspase levels was less obvious as compared to TNF-alpha treatment. We have also analyzed sensitivity of 17 breast cancer cell lines treated with radiation and calculated the corresponding ID50 of each cell line.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2002
- Accession Number
- ADA408160
Entities
People
- Xiaohe Yang