Understanding the Mechanism of Action of Breast Metastasis Suppressor BRMS1
Abstract
The focus of this study is to understand the biology behind the metastasis suppression via BRMS1, a recently identified metastasis suppressor gene. BRMS1 is a protein with a glutamic acid rich N-terminus, coiled-coil domain, an imperfect leucine zipper and nuclear localization signals. It is expressed almost ubiquitously in human tissues and is highly conserved across species. Sub- cellular fractionation and fluorescence immuno-cytochemistry has indicated that it localizes to nucleus. BRMS1 is shown to restore homotypic gap-junctional communication. Our hypothesis is that it may be involved in transcription regulatory complex. To identify proteins that interacting with BRMS1 a yeast two-hybrid screen was performed using full length BRMS1 as a bait and human mammary gland library as a prey. Eight genetic interactors of BRMS1 were identified. These are MRJ (Hsj40 related chaperon), CCG1 (a protein essential for progression of G phase), SMTN (cytoskelatal protein specific to smooth muscles), FLJ00052 (EST), KPNA5 (karyopherin alpha 5), Nmi (N-myc interactor), BAF 57(BRG1 associated factor) and RBP1 (Rb binding protein). The BRMS1 and RBP1 as well as BRMS1 and MRJ 1 interactions were further confirmed at cellular level by co-immunoprecipitation studies. Currently we are exploring the relevance of this interaction with respect to metastasis and cell cycle.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2002
- Accession Number
- ADA408646
Entities
People
- Rajeev S. Semant
Organizations
- Penn State College of Medicine