Direct Effects of Folate Metabolism on Gene Expression in Metastatic Breast Cancer
Abstract
Rab proteins are small GTPases that are essential elements of the protein transport machinery of eukaryotic cells. Each round of membrane transport requires a cycle of Rab protein nucleotide binding and hydrolysis. My research project consists in the study of Rab GTPases,the way in which they regulate intracellular transport, and the elucidation of mechanisms by which proteins involved in intracellular protein trafficking are linked to uncontrolled cellular proliferation and cancer. Our laboratory has extensively characterized Yiplp and Yoplp, membrane proteins which appear to play a role in Rab- mediated membrane transport in Saccharomyes cerevisiae. This past year I have further characterized other Rab interacting membrane proteins that are homologs of Yiplp. I have also cloned the mammalian homolog of Yiplp and initialed its characterization in mammalian cells. I will present exciting new evidence on the important role of prenylation in Rab protein localization and function and a potential role for Yiplp as a specific factor that binds the di-geranylgeranylated Rab. The results will be discussed in terms of the relevance to treatment for human cancers.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2002
- Accession Number
- ADA408721
Entities
People
- Monica Calero
- Ruth N. Collins
Organizations
- Cornell University