Identification of Multiple Pathogenic Bacteria Using a DNA Microarray
Abstract
The primary technique currently used to detect biological agents is based on immunoassays. Although sensitive and specific, inununoassays may not produce accurate identification based on single target detection and cannot determine subtle differences in the genome. Gene arrays can hybridize multiple DNA targets simultaneously, and thus, have enormous potential for detection and identification of pathogens. In this study, pathogenic E. coli 0157:117-specific genes, nonpathogenic Kl2-specific genes, common E. coli genes, and negative control genes were PCR-amplified and printed onto the surface of glass slides. Further, Staphylococcus aureus, Streptococcus pneumoniae, and Neisseria Meningitidis specific genes were also printed. After labeled bacterial cDNA samples were hybridized with probes on the microarray, specific fluorescence patterns were obtained, enabling identification of pathogenic E. coli 0157:117, nonpathogenic E. coli K12, antibiotic-resistant strain, and three other pathogenic bacteria. Because multiple datapoints are accessed, we demonstrate that this array method is more efficient and accurate than a typical immunoassay, which detects a specific protein product.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2002
- Accession Number
- ADA408810
Entities
People
- Chi-fang Wu
- James J. Valdes
- Jennifer W Sekowski
- William E. Bentley
Organizations
- Edgewood Chemical Biological Center