Simplified Microneutralization Test for Serotyping Adenovirus Isolates
Abstract
Several microneutralization procedures for serotyping of human adenovirus have been published, however, these procedures were tedious and time consuming requiring determination of virus challenge dose prior to testing, use of serial dilution of antisera and a staining step to interpret results. We sought to evaluate a simplified microneutralization procedure using an empirically determined virus challenge dose, a single dilution of antisera and microscopic observation of characteristic cytopathic effect as a basis for interpreting results. A total of 1,742 isolates from throat swabs collected from military recruits with febrile respiratory disease were serotyped by this simplified microneutralization test. Of the isolates tested, 1,697 (97.4%) were successfully typed. Validation of 100 randomly selected adenovirus isolates was performed using colorimetric microneutralization as the standard. The simplified microneutralization test results highly correlated (96%) with the standard method, with sensitivities ranging from 96 to 100% for the different adenovirus serotypes. This simplified microneutralization was on average 10 days faster than the standard method. Based on simplicity and speed, the simplified microneutralization test is the laboratory method of choice for high volume serotyping requirements.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2001
- Accession Number
- ADA408858
Entities
People
- David P. Schnurr
- Gregory C. Gray
- Leta K. Crawford-mkisza
- Marietta D. Malasig
- Pulak R. Goswami
Organizations
- Naval Health Research Center