Towards the Batch Synthesis of Long DNA

Abstract

Production-scale synthesis of long DNAs with specific base sequences by conventional methods is generally infeasible due to error rates On the other hand, sticky-ended double-stranded (ds) DNA complexes can be joined into a single dsDNA complex with an enzyme called DNA ligase This idea offers a way to indirectly synthesize very long (>10kb) DNAs-by hybridization of a batch of single-stranded (ss) DNAs followed by ligation-with predetermined base sequences We analyze such synthesis protocols In the natural course of this development, we take a reasonably self-contained tour through much of what is known about the physics of DNA hybridization as it relates to the issues here This paper is therefore a primer on DNA self-assembly, using the staggered ligation model for DNA synthesis as the working example

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2002
Accession Number
ADA409078

Entities

People

  • Steven Huntsman

Organizations

  • Institute for Defense Analyses

Tags

Communities of Interest

  • Energy and Power Technologies

DTIC Thesaurus Topics

  • Chemical Kinetics
  • Chemical Reactions
  • Chemical Synthesis
  • Chemistry
  • Computational Science
  • Deoxyribonucleic Acids
  • Differential Equations
  • Enthalpy
  • Heat Energy
  • Kinetic Energy
  • Mathematics
  • Nucleic Acids
  • Physical Chemistry
  • Random Variables
  • Self Assembly
  • Sequences
  • Thermodynamics

Fields of Study

  • Chemistry

Readers

  • Molecular Genetics
  • Systems Analysis and Design