Development of an EBV-Mammary Epithelial Cell Model for Addressing the Role of EBV in Breast Cancer
Abstract
In this grant we proposed to generate an EBV infected mammary epithelial system that could be used to characterize the life cycle and oncogenic properties of EBV in this unique tissue. We have been utilizing primary human mammary epithelial cell lines generated in Myles Brown's laboratory. By Fluorescence Activated Cell Sorting (FACS), we determined that these cells are positive for the EBV receptor, CR2 and we have been carrying out infection studies to determine the infectability of these cells to SBV. To date we have found that despite the presence of the CR2 receptor, infectibility is relatively low. This makes transient studies more difficult. We have therefore, begun generating long term cultures of infected IMEC cells through a variety of different means. We believe that we are making encouraging progress but we estimate that the completion of this project will require another year to complete. We have therefore obtained a 1 yr no cost extension to allow us to complete this work.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2002
- Accession Number
- ADA409341
Entities
People
- Erik K Flemington
Organizations
- Tulane University of Louisiana