Identification of Hepatocyte Growth Factor Autocrine Loops in Breast Carcinomas: Possible Target for Therapeutic Intervention

Abstract

Hepatocyte growth factor (HGF) binding to its transmembrane receptor, Met, results in an increase in breast epithelial cell motility, morphogenesis and metastasis. HGF-like forms have been detected as secretion products of the human breast epithelial carcinoma cell line, MCF10A1T3B. The two major products are ^56 and ^32 kDa. Recombinant HGF treatment with elastase produced the ^56 kDa HGF antagonistic product, an NK4-like form. Anion exchange chromatography of conditioned medium indicated that MCF10A1T3B secretion products have decreased affinity compared to endogenous full-length HGF.

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Document Details

Document Type
Technical Report
Publication Date
Aug 01, 2002
Accession Number
ADA409412

Entities

People

  • Bruce E. Elliot
  • Theodore G. Wright

Organizations

  • Queen's University

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Amino Acids
  • Biomedical Research
  • Breast Cancer
  • Cancer
  • Cell Line
  • Cell Movement
  • Cells
  • Diseases And Disorders
  • Epithelial Cells
  • Genetics
  • Growth Factors
  • Identification
  • Inhibitors
  • Intervention
  • Neoplasms
  • Peptides

Readers

  • Breast cancer cell signaling and growth regulation.
  • Molecular Biology and Genetics
  • Molecular and Cellular Biochemistry