Molecular Markers of Metastasis in Ductal Mammary Carcinoma
Abstract
The clinical outcome is generally positive for patients with node-negative breast carcinoma. In about 13% of those patients, however, the disease spreads, and they are at risk of death. Our goal is to develop DNA markers that could be reliably used to identify the ductal mammary carcinomas that are prone to develop metastasis. We compared DNA from normal cells and metastatic cells and also primary tumor cells and metastatic cells by representational difference analysis (RDA) method. We have isolated 15 metastasis associated DNA sequences (MADS), of which 3 were found be associated with metastasis in breast cancer patient samples other than the index case that was used in RDA experiments. Screening of primary tumors using MADS as fluorescence in situ hybridization (FISH) probes showed that MADS-IX is homozygously lost in some of the tumor cells (3/50) of a primary tumor that had positive lymph nodes where as it is not homozygously lost in any of the tumor cells (0/50) in the primary tumor that did not develop metastasis. Screening of additional cases is underway to determine if these results will be statistically significant.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2002
- Accession Number
- ADA409427
Entities
People
- Patnala Achary
Organizations
- Albert Einstein College of Medicine