Development of a Drug Targeting Approach for Cancer Therapy: Drug Carrier-Protein Conjugate
Abstract
Targeted delivery of anticancer drugs is one of the most actively pursued goals in anticancer chemotherapy A major disadvantage of anticancer drugs is their lack of selectivity for tumor tissue, which causes severe side effects and results in low cure rates, Any strategy by which a cytotoxic drug is targeted to the tumor, thus increasing the therapeutic index of the drug, is a way of improving cancer chemotherapy and minimizing systematic toxicity. This study covers the preparation of the gelatin microsphere (GM)- albumin (BSA) conjugate for the development of a "drug targeting" approach in cancer therapy. Gelatin microspheres of 5% (w/v) gelatin content were prepared by crosslinking with glutaraldehyde (GTA) at 0,5% (v/v) concentration, The particle size and morphology of microspheres were analyzed by Particle Size Analyzer and Scanning Electron Microscopy (SEM). Gelatin microspheres were chemically conjugated to bovine serum albumin PBS (O.O1M, pH 7.4) at 40C, The solution was then filtered and the GM-BSA conjugates were vacuum dried, The activity of BSA conjugated to GM was tested by using Immunodiffusion Techniques. The percent antigen-antibody binding between BSA and anti-BSA was evaluated by measuring the band widths of precipitates formed in the agar medium.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 25, 2001
- Accession Number
- ADA409706
Entities
People
- Asli Muvaffak
- Ismet Gurhan
- Nesrin Hasirci
- Ufuk Gunduz
Organizations
- Middle East Technical University