In Vivo Magnetic Resonance Imaging of Catheter-Based Vascular Gene Transfer

Abstract

The purpose of this study was to develop an in vivo imaging tool to monitor vascular gene transfer, We produced gadolinium/blue-dye and gadolinium/gene-vector media by mixing Magnevist with a trypan-blue or a lentiviral vector carrying a green fluorescent protein (CFP) gene, The gadolinium was used as an imaging marker for MRI to visualize vessel wall enhancement, while the blue-dye/CFP was used as a tissue stain marker for histology/immunohistochemistry to confirm the success of the transfer. Using Remedy gene delivery catheters, we transferred the gadolinium/blue-dye (n=8) or gadolinium/GFP-lentivirus (n=4) into the arteries of 12 pigs, monitored under high-resolution MR imaging This technical development enabled dynamic visualization of: (i) where the gadolinium/genes distributed; (ii) how satisfactorily the target portion was marked; and (iii) whether the gene transfer procedure caused complications. Our study represents the first direct evidence that catheter-based vascular gene delivery/distribution can be monitored by MR imaging in vivo.

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Document Details

Document Type
Technical Report
Publication Date
Oct 25, 2001
Accession Number
ADA410035

Entities

People

  • Dafu Wang
  • Dapeng Li
  • E. Atalar
  • J. M. Serfaty
  • Xiaoxuan Yang

Organizations

  • Johns Hopkins Hospital

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Arteries
  • Azo Compounds
  • Cardiovascular Diseases
  • Cardiovascular System
  • Diseases And Disorders
  • Gene Delivery
  • Gene Therapy
  • Health Services
  • High Resolution
  • Histology
  • Imaging Techniques
  • Magnetic Resonance
  • Magnetic Resonance Imaging
  • Therapy
  • United States
  • X Rays

Readers

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  • Medical Imaging.
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