Indicator Based and Indicator - Free Electrochemical DNA Biosensors

Abstract

The utility and advantages of an indicator free and MB based sequence specific DNA hybridization biosensor based on guanine and adenine oxidation signals and MB reduction signals have been demonstrated. Indicator free detection system is simple, cost-effective and provides rapid detection. The application of DPV at CPE fulfilled the expectations for the direct detection of hybridization between the known oligonucleotides. This procedure can be employed to detect specific gene sequences related to different viruses, bacterias or even inherited diseases. Improving the immobilization and hybridization steps through the use of covalent attachment schemes or PNA probes should further minimize non-specific adsorption effects and maximize sensitivity and speed. Reports are in progress towards these directions.

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Document Details

Document Type
Technical Report
Publication Date
Oct 25, 2001
Accession Number
ADA410061

Entities

People

  • Burcu Meric
  • Dilsat Ozkan
  • Kagan Kerman
  • Mehmet Ozsoz
  • Pinar Kara

Organizations

  • Ege University

Tags

Communities of Interest

  • Sensors

DTIC Thesaurus Topics

  • Analytical Chemistry
  • Biosensors
  • Chemical Reactions
  • Chemical Synthesis
  • Chemistry
  • Coordination Complexes
  • Detection
  • Dna Biosensors
  • Electrodes
  • Electron Transfer
  • Engineering
  • Hepatitis
  • Methylene Blue
  • Nucleic Acids
  • Oxidation
  • Polymerase Chain Reaction
  • Redox Indicators

Readers

  • Electrochemical Surface Science
  • Molecular and Cellular Biochemistry
  • Sensor Fusion and Tracking Systems.

Technology Areas

  • Biotechnology