An Epithelial-Derived, Integral Membrane, Kunitz-Type Serine Protease Inhibitor in Breast Cancer
Abstract
In the current research plan, we proposed to study the anti-tumor and anti-protease activity of a membrane- bound Kunitz-type serine protease inhibitor (KSPI; also known as HAI-1). In order to determine the effects of HAI-1 expression on breast cancer cell biology, we have transfected breast cancer cells MDA MB 435 cells with HAI-1 and selected 6 stable clones. In the first year, we have characterized these HAI-1 overexpressing breast cancer cells in vitro and in vivo, comparing to their parental cells and neo control. HAI-1 expression profoundly reduced the proliferation and in vivo tumor growth of MDA MB-435 cells. Additionally, HAI-1 expression altered the cellular morphology of this cell line, but had no effect on the cell motility. These results indicate that HAI-1 expression may inhibit tumor progression by modulating tumor growth, in a manner that is dependent upon the serine proteases, their substrates, and substrate effector molecules expressed by individual breast cancer cells.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2002
- Accession Number
- ADA410178
Entities
People
- Chen-yong Lin
Organizations
- Georgetown University