Gangliosides During Tumor Progression in Patients With Prostate Cancer
Abstract
Objectives of the project are: (1) to identify the gangliosides of Prostate cancer (CaP) cells that are immunogenic so that they can be used as targets to develop immunotherapy for prostate cancer; (2) to determine the total and specific Cap-gangliosides released into the blood and (3) to assess the nature of immunosuppression induced by CaP gangliosides. The major findings of the first year are as follows: Ganglioside GM1 is the major cell surface ganglioside of normal prostate epithelia. The expression of GM1 is significantly lowered in Prostate cancer cell lines (PC-3, DU145, LNCaP-FGC-10, LNCaP-FGC and HH870). On the other hand, the following gangliosides are more prominent: GM2>GD1b > GT1b (in all cell lines). GD1a is the highly expressed in PC-3 & DU145 cell lines. The immunogenicity of the CaP gangliosides was tested by comparing the serum antibody titers of healthy individuals and CaP-patients against eight different gangliosides. The gangliosides immunogenic in patients are GD1a> GT1b > GM2 > GD1b. The results enable us to identify the pathway of biosynthesis of prostate carcinoma associated gangliosides. The immunogenicity of the gangliosides suggests that they are potential targets for immunotherapy of Cap. The results lead to formulation of allogeneic CaP-vaccine with CaP-gangliosides.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2002
- Accession Number
- ADA410181
Entities
People
- Mepur H. Ravindranath