An AFM-Based Study of Porcine OBP Film for Artificial Olfaction

Abstract

The odorant binding protein (OBP) plays an important role in olfactory signal transmit. This paper studied the potential application of OBP on bionic artificial nose. Above all, OBP is immobilized in solid phase. So two kinds of OBP film were prepared. Firstly, OBP is covalently immobilized on the silicon wafer using DITC as a crosslinker. Secondly, polypyrrole film was fabricated on the ITO glass by the electrochemical polymerization. The topograph of these films was measured using Atomic Force Micrograph (AFM). In order to evaluate the affinity of OBP and its antibody, the AFM probe was coated with the anti-OBP using the covalent method. The force-distance curve, acquired by the AFM instrument, was analyzed to reveal the characteristic of thin film and the binding property between OBP and antibody.

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Document Details

Document Type
Technical Report
Publication Date
Oct 25, 2001
Accession Number
ADA410194

Entities

People

  • Guang Li
  • Min Pan
  • Qiang Cai
  • Yuquan Chen

Organizations

  • Zhejiang University

Tags

Communities of Interest

  • Advanced Electronics

DTIC Thesaurus Topics

  • Antibodies
  • Biomolecules
  • Biosensors
  • Carrier Proteins
  • Chemistry
  • Conductive Polymers
  • Detection
  • Electrochemical Cells
  • Electron Density
  • Engineering
  • Films
  • Fluorescence
  • Polymeric Films
  • Polymers
  • Proteins
  • Schematic Diagrams
  • Spectroscopy

Readers

  • Immunology
  • Nanoscale Plasmonic Nanotechnology
  • Thin Film Deposition Science.

Technology Areas

  • Biotechnology