The Role of the Tyrosine Kinase JAK2 in the Development and Progression of Breast Cancer
Abstract
The ongoing purpose of this work is to assess the contribution of the tyrosine kinase Jak2 in the development and progression of breast cancer. Using a mammary gland transplantation approach, it has been demonstrated that Jak2 is required for pregnancy- mediated proliferation and differentiation of mammary epithelium as assessed by electron microscopy and immunohistochemical analyses. Experiments are currently underway to generate a conditional mouse model in which the Jak2 gene can be specifically inactivated in mammary epithelium. Analyses of tumors arising in a mouse model with mutations in the Brca1 and p53 genes have revealed that the nuclear localization of Stat5a and Stat3 is heterogeneous both within tumors and between individual tumors. To further determine the possible role of Stat5a in human breast cancer, we have utilized human tissue arrays. Similar to the mouse model examined, heterogeneous nuclear and cytoplasmic Stat5a and Stat3 were observed. Finally, we have documented for the first time that the water transporter, aquaporin 5 (AQP5) and the Na-K-Cl cotransporter, NKCC1, are highly expressed in Brca1/p53 mutant mice suggesting they may be useful as prognostic markers.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2002
- Accession Number
- ADA410252
Entities
People
- Jonathan M. Shillingford