Examination of the Contribution of VEGF to the Metastatic Dissemination of c-Myc Overexpressing Breast Cancer Cells

Abstract

Metastasis to the lung is a common occurrence accounting for approximately 60-70% of metastasis. We have developed a transgenic model of lung metastasis using a MMTV-c-myc/MMTV-VEGF bitransgenic mouse system. We are using this system to evaluate the mechanism by which VEGF is able to dramatically increase metastasis to the lung. In spite of technical difficulties that have resulted in delays in the study, we have made significant progress towards generating the samples required to complete the proposed work and have developed a new series of cell lines for the project. Using these reagents we will evaluate the importance of blood vessel morphology and biology, versus tumor behavior in studies of the determinants of primary tumor intravasation and the estravasation of the shed cells at the site of the establishment of new metastatic deposits. Two papers describing our work will shortly be submitted for publication.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2002
Accession Number
ADA410255

Entities

People

  • Michael D. Johnson

Organizations

  • Georgetown University

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Biological Staining And Labeling
  • Biomedical Research
  • Blood
  • Blood Vessels
  • Breast Cancer
  • Cancer
  • Cell Line
  • Cells
  • Culture Techniques
  • Growth Factors
  • Mammary Glands
  • Medical Personnel
  • Metastasis
  • Neoplasms
  • Proteins
  • Tissues

Readers

  • Computational Modeling and Simulation
  • Oncology (Cancer Research).