Novel Angiogenic Domains: Use in Identifying Unique Transforming and Tumor Promoting Pathways in Human Breast Cancer
Abstract
We have now altered our strategy relative to the library to be used in these experiments. We now plan to use a cDNA library obtained from the National Cancer Institute. It is a cDNA library derived from four independent primary human breast cancers. This library is an exceedingly well-made library by individuals interested in breast cancer and experienced in making cDNA libraries. It is a composite of four separate tumors, providing the potential for discovery of additional genes that will cooperate with pleiotrophin to convert the MCF 1OA cells expressing the Ptn gene into a more aggressive growth phenotype. Furthermore, the library is not derived from a cell line, but rather is derived from primary tissues, thereby avoiding the problems of mutations introduced in cell lines through prolonged passage. This library has been obtained in the laboratory. We also have altered our strategy to now attempt to place this library in a retrovirus system. The transfection efficiency of this library will be considerably higher than the transforming efficiency or the system previously planned and, potentially, may bypass the need for selection.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2002
- Accession Number
- ADA410353
Entities
People
- Thomas F. Deuel
Organizations
- Beth Israel Deaconess Medical Center