The Tissue Transglutaminase Activity Does Not Strengthen Cell Adhesion During Mechanical Stretch

Abstract

Tissue transglutaminase activity (tTGA) has been shown to be important in cell adhesion to extracellular matrix (EM). However it is not well know if tTGA improves cell adhesion during mechanical strains imposed on the EM. We have compared the adherence properties of NIH 3T3-fibroblast cell line on fibronectin (FN) and on an EM that lacks the 42KD tTG-binding site. The cells were plated on silicone membranes and were stretched 10% also. Results indicate cell loose their morphology in the absence of tTG binding site and mechanical stretch exacerbates this poor response. The presence of tTGA is important in initial cell adhesion but does not strengthen the cell adhesion to EM.

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Document Details

Document Type
Technical Report
Publication Date
Oct 25, 2001
Accession Number
ADA410369

Entities

People

  • Mustafa Karamanoglu

Organizations

  • St Vincent's Hospital

Tags

DTIC Thesaurus Topics

  • Adhesion
  • Anti-Bacterial Agents
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Cellular Structures
  • Data Science
  • Fibroblasts
  • Health Services
  • Information Science
  • Integrins
  • Linear Regression Analysis
  • Membranes
  • Multivariate Analysis
  • Regression Analysis
  • Statistical Analysis
  • Substrates

Fields of Study

  • Biology

Readers

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